ABO Phenotypes, Rhesus and Kell2 antigens of Blood donors attending University of Calabar Teaching Hospital

Authors

  • Etura JE University of Calabar
  • Effiong UI University of Calabar
  • Asemota EA University of Calabar
  • Okoroiwu HU University of Calabar

DOI:

https://doi.org/10.60787/tnhj-24-1-658

Keywords:

Rh D, Cellano, Kell 2, Kell antigen, ABO, blood group, Rh antigen

Abstract

Background: Modern blood transfusion entails the transfusion of compatible blood units from a healthy donor to the recipient (patient). This study was aimed at evaluating rare and high- frequency blood antigens to aid planning in the study location.

Method: Descriptive cross-sectional study with systematic random sampling sample was employed in this study. The samples were analysed using commercially prepared reagents via serological technique (Standard tube agglutination method). The ABO was performed using anti-A, anti-B, and anti-AB reagent while Rh D was performed using anti D monoclonal reagent. KELL 2 (k) was performed using anti-kell reagent.

Result: Out of the 100 blood donors tested, 28 (28%) were KELL 2 (k+) positive while 100 (100%) were positive for Rh D antigen. ABO phenotype O constituted the majority (70%) of the studied subjects while A, B, and AB constituted 15%, 13%, and 2% respectively. The ABO cum Rhesus and blood group were in the order 0+> A+>, B+>, AB+.

Conclusion: KELL 2 antigen was relatively low among the studied subjects, while Rh D antigen was found to be a high-frequency antigen. Blood group O Rh D positive was found to be the most predominant blood group recorded among the studied participants.

References

Mitra R, Mishra N, Rath GP. Blood group systems. Indian J Anaesth. 2014; 58(5): 524-528.

Etura JE, Amaechi RA, Akpotuzor JO, Okoroiwu HU. Demographics of Rhesus phenotype of blood donors in Calabar: A case study of University of Calabar Teaching Hospital, Calabar, Cross River State, Nigeria. Advances in Hematology. 2020: ID 2659398. https://doi.org/10.1155/2020/2659398.

International Society of Blood Transfusion (ISBT). Red cell Immunogenetics and blood group terminology. Available at :https://www.isbtweb.org/isbt-working-parties/vcibgt.html. Accessed May 1, 2022.

Dean L. Blood groups and red cell antigens. National center for Biotechnology Information (US). 2005. Chapter 5. The ABO Blood group Available at: https://www.ncbi.nlm.nih.gov/books/NBK2267/

Wethorff CM. The Rh blood system in review: A new face for the next decade. Transfusion. 2004; 44: 1663-73.

Wiler M. The Rhesus blood group system. In: Modern Blood Banking and Transfusion Practices. Haimening DM (ED). 3rd edition. Jaypee Medical Publishers, India. 1998: 116-132.

Wagner FF, Frohmajer A, Ladenig B, et al. Weak D alleles express distinct phenotypes Blood. 2000;95(8): 2699-2708.

David-Wert AS. Blood transfusion and blood management in a tropical country. Clinics in Hematology. 1981; 10(3): 1013-1023.

Dean L. Blood Groups and Red Cell Antigens. National Center for Biotechnology Information (US). 2005. Chapter 8: The Kell blood group. Available from: https://www.ncbi.nlm.nih.gov/books/NBK2270/

Gassner C, Degenhardt F, Meyer S, Voumeit C, et al. Low frequency blood group antigen in Switzerland. Transfusion Medicine and Hemotherapy. 2018; 45: 239-250.

Harmening D. Modern blood banking and transfusion practices. 6th ed. Philadelphia. FA Davis. 2012; 199.

Mahmood A, Alam M, Yazdani MS, Rathore MA. Frequency of Kell antigens (K & K) among blood donors of Northern Pakistan. Pak Armed Forces Med J. 2019; 69(5): 977- 80.

Woodfield G, Poole J, Nance ST, Daniel G. A review of the ISBT rare blood donor program. Immunohematology. 2004; 20: 244 – 248.

Ekere EF, Useh MF, Okoroiwu HU, Mirabeau TY. Cystein-CysteinChemokine receptor 5 (CCR5) profile of HIV -infected subjects attending University of Calabar Teaching Hospital, Calabar, Southern Nigeria. BMC Infectious Disease. 2020; 20:5. DOI: https://doi.org/10.1186/s12879-019-4737-1.

Okoroiwu HU, Uchendu KI, Essien RA (2020) Causes of morbidity and mortality among patients admitted in a tertiary hospital in southern Nigeria: A 6-year evaluation. PLoS ONE 15(8): e0237313.

Adewoyin AS, Lee GM, Adeyemo TA, Awodu OA.Rh and Kell blood Group antigen Prevalence in a multi-ethnic cohort in Nigeria: Implication for local transfusion service. Immunohematology. 2018; 34(2): 61 – 65.

Gwaram BA. And Yusuf BJ. A preliminary study on the prevalence of weak blood group antigens among blood donors in Aminu Kano Teaching Hospital, Kano, Nigeria. Dutse Journal of Pure and Applied Sciences. 2020; 6(2): 336 – 343.

Owaidah AY, Naffaa NM, Alumran A, Alzahrani F. Phenotype frequencies of major blood group systems (Rh, Kev, Kidd, Dully, MNS, P, Lewis, and Lutheran) among blood donors in the Eastern region of Saudi Arabia. Journal of Blood Medicine. 2020;11: 59 - 65.

Thakral B, Saluja K, Sharma RR, Marwaha N. Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Dully, MNS, P, Lewis, and Lutheran) in North Indian blood donors. Transfusion and Apheresis Science. 2010; 43 (1): 17 – 22.

Yu Y, Ma C, Sun X, Guan X, Zhang X, Saldanha J, Chen L, Wang D. Frequencies of red blood cell major blood group antigens and phenotypes in the Chinese Han population from Mainland China. International Journal of Immunogenetics. 2016; 43 (4): 226 – 235.

Bogui LS, Dembele B, Sekongo Y, Abisse S, Konate S, Sombo M. Phenotype profile of Rh and Kell blood group donors in Cote d’Ivoire, West Africa. Journal of Blood Transfusion. 2014; 309817. https://doi.org/10.1155/2014/309817

Al – Riyani AZ, Al – Marhoobi, Al – Hosni S, Al – Mahrooqi S, Schmidt M, O’brien S, and Al – Khabori M. Prevalence of red blood cell major blood group antigens and phenotypes among Omani blood donors. Oman Medical Journal. 2019; 34 (6): 496-503

Hameed A, Hussain W, Ahmed J, Rabbi F and Qureshi JA. Prevalence of phenotypes and genes of ABO and Rhesus (Rh) blood groups in Faisalabad, Pakistan. Pakistan Journal of Biological Science. 2002;5: 722 – 724.

Mehmood A, Alam M, Yazdani MS, Rathore MA. Frequency of Kell antigens (K & K) among blood donors of North Pakistan. 2019; 69 (5): 977- 80.

Reid ME and Lomas- Francis C. The blood group antigen fact book. New York: Elsevier Academic Press. 2004.

Okoroiwu HU, Asenota EA. Blood donor deferral prevalence and causes in a tertiary healthcare hospital, Southern Nigeria. BMC Health Service Research. 2019; 19: 510. Doi.org/10.1186/s12913-019-4352-2.

Adienbo OM, Nwafor A, Egwurugwu JN, Okon UA. The distribution of ABO and Rhesus blood group among indigenes of Ijaw ethnic group in Niger Delta region Nigeria. Global Journal of Pure and Applied Science. 2010; 16 (3): 345- 348.

Enosolease ME and Bazuaye GN. Distribution of ABO and Rh – D blood groups in the Benin area of Niger – Delta: Implications for regional blood transfusion. Asian J. Transfuse Sci. 2008; 2(1): 3 – 5.

Musa AU, Ndakotsu MA, Abdul – Aziz H, Kilishi A, Aliyu I. Distribution of ABO and Rhesus blood group systems among blood donors in Sokoto North – West Nigeria. J ApplHematol. 2015; 6: 136 – 138.

Chima OK, Mohammed TB, Aisha K, Alhaji SA, Muhammad BM, et al. ABO and Rhesus blood groups among blood donors in Kano, North – West Nigeria. Niger Basic Clin. Sci. 2012; 9: 11-13.

Ugwu NI. Pattern of ABO and Rhesus blood group distribution among students of Ebonyi State University, Abakaliki, South Eastern Nigeria. Asian Journal of Medical Sciences. 2016; 7 (1): 101 - 104.

Doku GN, Agbozor WK, Annor RA, Kisseh GD, Owusu MA. Frequency of ABO/Rhesus (D) blood groups and ethic Distribution in Great – Accra region of Ghana,towards effective blood bank inventory. Int J Immunogenetics. 2019; 46 (2): 67 – 73.

Ndoula ST, Noubiap JJN, Nansseu JRN, Wonkam. Phenotypic and allelic distribution of ABO and Rhesus (D) blood groups in the Cameroonian Population. Int Journal Immunogenetics. 2014; 41 (3): 206 – 10.

Sawadogo S, Nebie K, Milloyo T, Kafando E, Sawadogo A, et al. Distribution of ABO and Rh D blood group antigens in blood donors in Burkina Faso. Int J. Immunogenetics. 2018; 46 (1): 1 – 6.

Basu D, Dalta SS, Montemayor C, Bhattacharya P, Mukherjee K, et al. ABO, Rhesus and Kell antigens, alleles and haplotypes in West Bengal, India. Transfus. Med. Hemother. 2018; 45 (1): 62 – 66.

Eissa AA. ABO and Rh Bloodgroups polymorphism among the Kurds of Duhok, Iraq. Duhok Medical Journal. 2014; 8 (1): 1 – 6.

Sarhan MA, Saleh KA, Bin – Dajem SM. Distribution of ABO blood groups and rhesus factor in South West Saudi Arabia. Saudi. Med. J. 2009; 30 (1): 116 – 9.

Dilek I, Demir C, Bay A, Akdeniz H, Oner AF. ABO and Rhesus blood groups frequency in men and women living in eastern Turkey. UHOD UluslararasiHematolDerg. 2006; 16 (1): 23 – 26.

Okoroiwu HU and Okafor IM. Demographic Characteristics of blood and blood components transfusion recipients and pattern of blood utilization in a tertiary health institution in Southern Nigeria. BMC Hematology, 2018; 18 :16.

Cavalli-Sforza, LL, Piazza A. Consanguinity and genetic drift: Their combined effects on the ABO blood group frequencies in a small Italian population. American Journal of Human Genetics. 1974; 26(3), 417-434.

Agyei-Baffour, N. (2022). The role of natural selection in ABO blood group frequencies in Africa. Malaria Journal. 2022; 21(1): 301.

Rowe JA, Handel IG, Thera MA, Deans AM, Lyke KE, Koné A, Diallo DA, Raza A, Kai O, Marsh K, Plowe CV, Doumbo OK, Moulds JM. Blood group O protects against severe Plasmodium falciparum malaria through the mechanism of reduced rosetting. Proc Natl Acad Sci U S A. 2007 Oct 30;104(44):17471-6.

Yeda R, Okudo C, Owiti E. et al. Burden of malaria infection among individuals of varied blood groups in Kenya. Malar J 2022; 21:251.

Menozzi G, Piazza A, Cavalli-Sforza LL. Synthetic maps of gene frequencies in Italy. Am J Hum Genet 1970;22(3):329-348.

Nei M. Molecular Population Genetics. New York: John Wiley & Sons; 1975. 446.

Pritchard JK, Di Rienzo A, Wahl LM. The role of population structure and selection in shaping human genetic variation. Annu Rev Genet 2020; 54:1-35.

World Atlas. World Facts; highest number of sickle cell birth by country. [Internet]. Accessed April 23, 2023. Available from: https://www.worldatlas.com/articles/countries-with-the-highest-number-of-sickle-cell-births-per-year.html

Okoroiwu HU, Lopez – Munoz F, Povedano – Montero FJ. Bibliometric analysis of global sickle cell disease research from 1997 to 2017. Hematology, Transfusion and Cell Therapy. 2022; 44 (2): 186 – 196

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Published

2024-03-29

How to Cite

Etura, J. E., Effiong , U. I., Asemota, E. A., & Okoroiwu, H. U. (2024). ABO Phenotypes, Rhesus and Kell2 antigens of Blood donors attending University of Calabar Teaching Hospital. The Nigerian Health Journal, 24(1), 1010–1016. https://doi.org/10.60787/tnhj-24-1-658

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